Exploration of Active Metabolites from Aegle Marmelos and Emblica Officinalis for Determining its Immunosuppressive Properties

  • Amit Gupta
Keywords: Aegle marmelos, Emblica officinalis, proinflammatory, hepatitis B vaccine

Abstract

As per the literature, bioactive compounds isolated or purified from medicinal plant products that are generally used for curing various human diseases. The present study involves two different medicinal plants i.e. Aegle marmelos and Emblica officinalis were locally available in Baramati region of Maharashtra, India. The leaves of these medicinal plants were used for phytochemical analysis in aqueous extract using phosphate buffered saline (PBS; pH 7.2) in order to find out the active metabolites using liquid chromatography mass spectrometry (LC-MS). For these studies, aqueous leaves extract of these two medicinally plants were tested for immunological studies in mice model studies especially Swiss mice pertaining to determine splenocyte proliferation assay using hepatitis B vaccine containing surface antigen (HBsAg) and also determined pro-inflammatory cytokines (IFN-gamma and TNF alpha) by Elisa. The results showed that aqueous leaves extract showed declined in splenocyte proliferation assay and proinflammatory cytokines (FN-gamma and TNF alpha) at higher doses as compared to control samples. HBsAg (20 µg/ml) used as standard for these studies. Overall, the results showed that aqueous leaves extract of Aegle marmelos and Emblica officinalis showed immunosuppressive activity against HBsAg as compared to control.

Downloads

Download data is not yet available.

References

REFERENCES

Gupta A and Chaphalkar SR. Anti-inflammatory and immunosuppressive activities of flavonoids from medicinal plants. Journal of Herb Med Pharmacology 2016; 5 (3): 121 -124.

Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Roy C. Screening of Indian plants for biological activity. Part I. Indian J of Exp Biol 1968; 6: 232 – 247.

Gupta A, Khamkar PR and Chaphalkar SR. Review on medicinal plants to target and inhibit the epidermal growth factor receptor signalling in cancer and tissue repair therapy. International Journal of Advances in Pharmacy, Biology and chemistry 2014; 3(1): 210- 213.

Gupta A and Chaphalkar SR. Flow cytometry based assay of formulation from Syzygium cumini in human whole blood and glycosylated red blood cells. Journal of Pharma research 2014, 3 (12): 265 – 270.

Chaudhuri N, Pal A and Gomes S. Anti-Inflammatory and related action of Syzygium cumini Seed Extract. Phytotherapy Research 1990; 4(2): 5-10.

Gupta A, Shah AP, Jambhale AD, Choudhari V and Chaphalkar SR. Non- steroidal anti-inflammatory drugs: Overview. World journal of Pharmaceutical Sciences 2016; 4(1): 93 – 100.

Gillett NA and Chan C. Applications of immunohistochemistry in the evaluation of immunosuppressive agents. Human & Experimental Toxicology 2000; 19 (4): 251–254.

Gupta A and Chaphalkar SR. Anti-inflammatory and immunosuppressive activities of flavonoids from medicinal plants. Journal of Herb Med Pharmacology 2016; 5 (3): 121 -124.

Gupta A and Chaphalkar SR. Immunosuppressive activity of crude proteases from Ficus benghalensis on human whole blood using flow cytometry. Asian Journal of clinical research 2016; 2:1-5.

Gupta A and Chaphalkar SR. Immunosuppressive and cytotoxic potential of flavonoids from Mitragyna parvifolia, Mangifera indica and Aegle marmelos. Journal of Pharmacology and toxicological studies 2016; 4 (1): 1 – 5.

Gupta A and Chaphalkar SR. Flow cytometric analysis of immunoadjuvant activity of Emblica officinalis on human whole blood. World Journal of Pharmaceutical research 2015; 4 (2): 1063 - 1071.

Gupta A and Chaphalkar SR. Haemolytic activities and anti-diabetic effect of Terminalia arjuna and Emblica officinalis. European Journal of Pharmaceutical and medical research 2016; 3 (6): 334 – 338.

Khajuria A, Gupta A, Malik F, Singh S, Singh J, Gupta BD, Suri KA, Suden P, Srinivas VK, Ella K and Qazi GN. A new vaccine adjuvant (BOS 2000) a potent enhancer mixed Th1/Th2 immune responses in mice immunized with HBsAg. Vaccine 2007; 25 (23): 4586 – 4594.

Khajuria A, Gupta A, Singh S, Malik F, Singh J, Suri KA, Satti NK, Qazi GN, Srinivas VK, Gopinathan and Ella K. RLJ-NE-299A: A new plant based vaccine adjuvant. Vaccine 2007; 25 (14): 2706 – 2715.

Winzer T, Gazda V, He Z, Kaminski F, Kern M, Larson TR, Li Y, Meade F, Teodor R, Vaistij FE, Walker C, Bowser TA and Graham IA. Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine. Science 2012; 336 (6089): 1704-1708

Dai Y, DeSano J, Qu Y, Tang W, Meng Y, Lawrence TS and Xu L. Natural IAP inhibitor Embelin enhances therapeutic efficacy of ionizing radiation in prostate cancer, Am J Cancer Res 2011;1(2):128-143.

Swamy HMK, Krishna V, Shankarmurthy K, Rahiman BA, Mankani KL, Mahadevan KM, Harish BG and Naika HR. Wound healing activity of embelin isolated from the ethanol extract of leaves of Embelia ribes Burm. Journal of Ethnopharmacology 2007; 109 (3): 529–534

Singh B, Guru SK, Sharma R, Bharate SS, Khan IA, Bhushan S, Bharate SB and Vishwakarma RA. Synthesis and anti-proliferative activities of new derivatives of embelin. Bioorganic and Medicinal Chemistry Letters 2014; 24: 4865–4870.

Tan M. Granisetron: new insights into its use for the treatment of chemotherapy-induced nausea and vomiting. Expert Opin Pharmacother 2003; 4(9):1563-71.

Gupta A and Chaphalkar SR. Immunosuppressive terpenoids from extracts of Emblica officinalis, Ficus racemosa and Strychnos nux-vomica. Micromedicine 2016; 2 (1): 1 – 7.

Gupta A and Chaphalkar SR. Analytical studies of protease extracted from Azadirachta indica. World Journal of Pharmaceutical research 2015; 4 (11):1391 - 1398.

Gupta A and Chaphalkar SR. Flow cytometry based assay of formulation from Syzygium cumini in human whole blood and glycosylated red blood cells. Journal of Pharma research 2014; 3 (12): 265 - 270.

Gupta A and Chaphalkar SR. Inhibitory potential of aqueous extract of Ficus benghalensis on human peripheral blood mononuclear cells. Journal of Pharma research 2014; 3(11): 250 – 253.

Published
2016-08-25
How to Cite
Gupta, A. (2016). Exploration of Active Metabolites from Aegle Marmelos and Emblica Officinalis for Determining its Immunosuppressive Properties. Journal of Progressive Research in Biology, 3(1), 119-126. Retrieved from http://www.scitecresearch.com/journals/index.php/jprb/article/view/859
Section
Articles